Isothiazolone Reduced Microbe Contamination of North Sumatra Local Garlic (Allium sativum L.) in vitro

Abstract

Background and Objective: Sterilization of the explant is a very crucial stage in the tissue culture process because it determines the success to step into the process of induction of shoots or calluses. The purpose of this study was to get a sterile explant but does not suffer damage to plant cells or tissues due to sterile materials. Materials and Methods: The study used a Completely Randomized Design (CRD) factorial with 2 treatment factors. The 1st factor was the sterilization method with 3 methods consisting of P1 (clorox 20% for 30 min then soaked in a solution of peracetic acid 0.5% for 5 min), P2 (clorox 20% for 30 min then soaked in chloramphenicol 1000 ppm for 60 min) and P3 (clorox 20% for 30 min then soaked in isothiazolone 1.5 mL LG1 for 60 min). The second was the addition of isothiazolone to the media, namely M0 (medium MS without the addition of isothiazolone) and M1 (medium MS+1.5 mL LG1 isothiazolone). The observed variables were the percentage of live explants and the level of explants contamination. Results: The results showed that the percentage of explant life observed reached 100% in all treatments, while the sterilization method had a significant effect on the level of sterilization, but the media type treatment gave an insignificant response and there was an interaction between the sterilization method treatment and the type of media. Conclusion: The best treatment to eliminate the contamination of local garlic was P3M0.